Ambient pH signal transduction in Aspergillus: completion of gene characterization
نویسندگان
چکیده
منابع مشابه
Further characterization of the signaling proteolysis step in the Aspergillus nidulans pH signal transduction pathway.
The Aspergillus nidulans pH-responsive transcription factor PacC is modulated by limited, two-step proteolysis. The first, pH-regulated cleavage occurs in the 24-residue highly conserved "signaling protease box" in response to the alkaline pH signal. This is transduced by the Pal signaling pathway, containing the predicted calpain-like cysteine protease and likely signaling protease, PalB. In t...
متن کاملRegulation of gene expression by ambient pH in Aspergillus: genes expressed at acid pH.
Ambient pH strongly influences the synthesis of secreted enzymes, permeases and exported metabolites by a wide range of micro-organisms. For example, numerous microbes secrete acid phosphatase in acidic environments and alkaline phosphatase in alkaline environments. Only in the ascomycete Aspergillus nidulans, however, is characterization [ 1-51 of the pH-regulatory system sufficiently advanced...
متن کاملAspergillus nidulans Ambient pH Signaling Does Not Require Endocytosis.
Aspergillus nidulans (Pal) ambient pH signaling takes place in cortical structures containing components of the ESCRT pathway, which are hijacked by the alkaline pH-activated, ubiquitin-modified version of the arrestin-like protein PalF and taken to the plasma membrane. There, ESCRTs scaffold the assembly of dedicated Pal proteins acting downstream. The molecular details of this pathway, which ...
متن کاملMutational analysis of the pH signal transduction component PalC of Aspergillus nidulans supports distant similarity to BRO1 domain family members.
The alkaline ambient pH signal transduction pathway component PalC has no assigned molecular role. Therefore we attempted a gene-specific mutational analysis and obtained 55 new palC loss-of-function alleles including 24 single residue substitutions. Refined similarity searches reveal conserved PalC regions including one with convincing similarity to the BRO1 domain, denoted PCBROH, where clust...
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Phytochromes regulate various light responses through their interactions with different signaling proteins, such as PIF3. However, the physiological functions of PIF3 in light signaling are not yet fully understood. To increase our understanding of these roles, we characterized a T-DNA insertional pif3 mutant and transgenic plants overexpressing the full-length PIF3 . Transgenic overexpressing ...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1999
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.1999.01693.x